We present experimental results, which show that the up-chirp of dispersively stretched femtosecond pulses decreases linearly with increasing pulse energy after amplification in Erbium-doped fibers. For 6 μJ output pulses a nonlinear dispersion of -4 • 106 fs2 was measured. This nonlinear dispersive effect is attributable to the resonant dispersion of the Erbium-ions and the decrease of the inversion during pulse amplification and was about one order of magnitude larger than predicted by the literature. Most likely this deviation is attributable to the complex population dynamics of the Er3+-ions during pulse amplification, since in the literature the relation between the refractive index and Er3+-inversion was described for a quasi-static population distribution. Due to the high resonant dispersion the required compressor dispersion for minimum output pulse duration depends strongly on the output pulse energy in Erbium-doped fiber-based chirped-pulse-amplifier set-ups.