In the field of hearing research a~variety of imaging techniques are available to study molecular and cellular structures of the cochlea. Most of them are based on decalcifying, embedding, and cutting of the cochlea. By means of scanning laser optical tomography (SLOT), the complete cochlea can be visualized without cutting. The Cav1.3−/− mice have already been extensively characterized and show structural changes in the inner ear. Therefore, they were used in this study as a~model to investigate whether SLOT can detect structural differences in the murine cochlea.