Molecular delivery methods are key technologies in the biomedical sciences. Gold nanoparticle mediated (GNOME) laser transfection combines the high efficiency and low invasiveness of single cell laser transfection with a high sample throughput and easy implementation required for routine usage. It is based on the laser-induced excitation of membrane adhered gold nanoparticles, which leads to transient and spatially confined permeabilization of the cellular membrane. This allows the entry of extracellular molecules for the targeted manipulation of living cells. Herein, we describe an automated laser transfection device which provides easy usage and laser safety to end-users. Using this setup, the permeabilization parameters were extensively optimized to provide efficient delivery of molecules while maintaining a high cell viability of approximately 90\%. siRNA and Morpholino oligomer mediated knockdown by GNOME laser transfection with knockdown efficiencies up to 88\% were achieved. Furthermore, protein injection of fluorescent human serum albumin was demonstrated. The results demonstrate the applicability of GNOME laser transfection for routine transfection and high throughput screening purposes.